HiPrep Sephacryl S-200 HR (high-resolution) size exclusion chromatography columns are designed for routine protein purification at laboratory scale.
- Broad fractionation range: Mr ~ 5 000 to ~ 250 000 (globular proteins), Mr ~ 1 000 to ~ 80 000 (dextrans) suitable for varied applications, including purification of recombinant tagged proteins.
- High capacity columns: Preparative separation of protein in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively.
- Convenient: Pre-packed Sephacryl columns are easily connected to ÄKTA systems and other chromatography systems for automated chromatography at low to medium pressure.
Fast and reproducible, for routine protein purification
Sephacryl resins are dextran-acrylamide copolymers with extensive cross-linking, which gives good rigidity and chemical stability. The high resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl resins an excellent option for routine purification by size exclusion chromatography (SEC). SEC is also known as gel filtration chromatography.
